Product code: RXSOL-60-6604-353
COOMASIE BRILLANT BLUE G 250
Coomassie Brilliant Blue G-250 (CBBG) is a member of the Coomassie family of,
triphenylmethane dyes and is used as a common analytical dye in SDS-PAGE and BN-PAGE.
CBBG can also be used with the Bradford Method to determine protein concentration.
Application
Coomassie Brilliant Blue R-250 was first used to visualise proteins in 1964 by Fazekas de St. Groth and colleagues. Protein samples were separated electrophoretically on a cellulose acetate sheet. The sheet was then soaked in sulfosalicylic acid to fix the protein bands and then transferred to a solution of the dye.